BTAF1 polyclonal, anti-human, mouse, rat

Background:
TFIID is a general transcription factor which initiates preinitiation complex assembly through direct interaction with the TATA promoter element. It is a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs). Although native TFIID can mediate both activator-independent (basal) and activator-dependent transcription in reconstituted systems, TBP can mediate only basal transcription. The largest subunit (TAF) of TFIID is a protein designated TAF II p250. B-TFIID is an initial factor composed of TBP and TAF II p170 that has been identified as a pol II transcription factor. TAF II p170 has been shown to have potent (d)ATPase activity.

Alternative Name:
TATA-binding protein-associated factor 172, ATP-dependent helicase BTAF1, B-TFIID transcription factor-associated 170 kDa subunit, TAF(II)170, TBP-associated factor 172, TAF-172, TAF172

Application Dilution: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IP: 1:20 - 1:50, ChIP: 1:20 - 1:100

Specificity: BTAF1 polyclonal antibody detects endogenous levels of BTAF1 protein.

Immunogen:
Recombinant full length Human BTAF1.

MW: ~ 170, 206 kDa

Swis Prot.: O14981

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.