BUB1 (F818) polyclonal, anti-human
€305.00
In stock
SKU
BS9180
Background:
Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.
Alternative Name:
Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1A, BUB1, BUB1L
Application Dilution: WB: 1:500~1:1000
Specificity: BUB1 (F818) polyclonal antibody detects endogenous levels of BUB1 protein.
Immunogen:
Synthetic peptide, corresponding to amino acids 791-840 of Human BUB1.
MW: ~ 122 kDa
Swis Prot.: O43683
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.
Alternative Name:
Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1A, BUB1, BUB1L
Application Dilution: WB: 1:500~1:1000
Specificity: BUB1 (F818) polyclonal antibody detects endogenous levels of BUB1 protein.
Immunogen:
Synthetic peptide, corresponding to amino acids 791-840 of Human BUB1.
MW: ~ 122 kDa
Swis Prot.: O43683
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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