Calpain 1 polyclonal, anti-human, mouse, rat

Background:
Calpain 1, also designated µ-calpain, is an intracellular calcium-dependent protease that cleaves cyto-skeletal and submembranous proteins. Calpains are nonlysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+-dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpain-1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Alternative Name:
Ca2 activated neutral protease, Calcium activated neutral proteinase 1, Calcium activated neutral proteinase, Calcium activated neutral proteinase small subunit, Calcium dependent protease small subunit 1, Calcium dependent protease small subunit, Calcium-activated neutral proteinase 1, Calpain 1 (mu/I) large subunit, Calpain 1, Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain Large Polypeptide L1, Calpain Large Polypeptide L1, Calpain mu type, Calpain mu-type, Calpain regulatory s

Application Dilution: WB: 1:500-1:2000, ICC: 1:50-1:200, IHC: 1:50-1:200, FCM: 1:100

Specificity: Calpain 1 Antibody detects endogenous levels of total Calpain 1.

Immunogen:
A synthesized peptide derived from human Calpain 1.

MW: ~ 82 kDa

Swis Prot.: P07384

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.