CD3 FITC / CD16+56 PE / CD45 PerCP-Cyanine5.5 / CD4 PE-Cyanine7 / CD19 APC RUO
€920.00
In stock
SKU
IS-TBNK2
Introduction:
TCD3 (clone 33-2A3) recognizes the ε-chain of the CD3/TCR complex, which is expressed on all mature T lymphocytes. This antigen is a defining marker of the T-cell lineage and is essential for T-cell receptor signaling. CD3 expression is absent on B cells, NK cells, and other leukocyte subsets, allowing for specific identification of T cells by flow cytometry.
CD4 (clone SK3) identifies a glycoprotein expressed predominantly on helper/inducer T lymphocytes. CD4 acts as a co-receptor for MHC class II molecules and plays a central role in T-cell activation and immune regulation. It is also found at low levels on monocytes.
CD8 (clone 143-44) recognizes a cell surface glycoprotein that interacts with MHC class Imolecules. It is expressed mainly on cytotoxic/suppressor T lymphocytes and a subset of NK cells. CD8 enhances adhesion to target cells and contributes to T-cell activation.
CD16 (clone 3G8) binds to the low-affinity FcγRIII receptor for IgG, which is present on NK cells, neutrophils, and some monocyte subsets. It is widely used to identify NK cells in combination with CD56, although it can also label granulocytes.
CD56 (clone B-A19) targets the neural cell adhesion molecule (NCAM), which is expressed on natural killer (NK) cells and a subset of T lymphocytes (CD3⁺CD56⁺ NKT-like cells). CD56 is essential for the identification of NK populations in peripheral blood.
CD45 (clone D3/9) binds to all isoforms of the leukocyte common antigen (LCA), which is present on all human leukocytes, including lymphocytes, monocytes, granulocytes, eosinophils, and basophils. CD45 is used to distinguish leukocytes from non-hematopoietic cells and debris.
CD19 (clone A3-B1) recognizes a transmembrane protein expressed throughout B cell development, excluding plasma cells. It is a pan-B cell marker that does not react with T cells, monocytes, or granulocytes, allowing for specific detection of B lymphocytes.
Recomended usage: TBNK reagent panel, which includes the combinations CD3 FITC / CD4 PE-Cyanine7 / CD8 APC-Cyanine7 / (CD16+CD56) PE /CD45 PerCP-Cy5.5 / CD19 APC and CD3 FITC /(CD16+CD56) PE / CD45 PerCP / CD19 APC, is intended for in vitro diagnostic use for the qualitative identification of major lymphocyte subsets — T cells (CD3, CD4, CD8), B cells (CD19), and NK cells (CD16+CD56) — in human peripheral blood samples by flow cytometry.
This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 10 6 cells.
Application: FCM
Storage Temperature: Store in the dark at 2-8 °C
Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed (at least, one year)
TCD3 (clone 33-2A3) recognizes the ε-chain of the CD3/TCR complex, which is expressed on all mature T lymphocytes. This antigen is a defining marker of the T-cell lineage and is essential for T-cell receptor signaling. CD3 expression is absent on B cells, NK cells, and other leukocyte subsets, allowing for specific identification of T cells by flow cytometry.
CD4 (clone SK3) identifies a glycoprotein expressed predominantly on helper/inducer T lymphocytes. CD4 acts as a co-receptor for MHC class II molecules and plays a central role in T-cell activation and immune regulation. It is also found at low levels on monocytes.
CD8 (clone 143-44) recognizes a cell surface glycoprotein that interacts with MHC class Imolecules. It is expressed mainly on cytotoxic/suppressor T lymphocytes and a subset of NK cells. CD8 enhances adhesion to target cells and contributes to T-cell activation.
CD16 (clone 3G8) binds to the low-affinity FcγRIII receptor for IgG, which is present on NK cells, neutrophils, and some monocyte subsets. It is widely used to identify NK cells in combination with CD56, although it can also label granulocytes.
CD56 (clone B-A19) targets the neural cell adhesion molecule (NCAM), which is expressed on natural killer (NK) cells and a subset of T lymphocytes (CD3⁺CD56⁺ NKT-like cells). CD56 is essential for the identification of NK populations in peripheral blood.
CD45 (clone D3/9) binds to all isoforms of the leukocyte common antigen (LCA), which is present on all human leukocytes, including lymphocytes, monocytes, granulocytes, eosinophils, and basophils. CD45 is used to distinguish leukocytes from non-hematopoietic cells and debris.
CD19 (clone A3-B1) recognizes a transmembrane protein expressed throughout B cell development, excluding plasma cells. It is a pan-B cell marker that does not react with T cells, monocytes, or granulocytes, allowing for specific detection of B lymphocytes.
Recomended usage: TBNK reagent panel, which includes the combinations CD3 FITC / CD4 PE-Cyanine7 / CD8 APC-Cyanine7 / (CD16+CD56) PE /CD45 PerCP-Cy5.5 / CD19 APC and CD3 FITC /(CD16+CD56) PE / CD45 PerCP / CD19 APC, is intended for in vitro diagnostic use for the qualitative identification of major lymphocyte subsets — T cells (CD3, CD4, CD8), B cells (CD19), and NK cells (CD16+CD56) — in human peripheral blood samples by flow cytometry.
This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 10 6 cells.
Application: FCM
Storage Temperature: Store in the dark at 2-8 °C
Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed (at least, one year)
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