ExoELISA-Step human Serum/Plasma RUO
€695.00
In stock
SKU
IS-Exo2508
Introduction:
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB) with the plasma membrande. They are thought to provide a means of intercellular communication and of transmission of macromoleculaes between cells allowing the spread of lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.
Product Description:
ExoELISA-Step is an assay based on the use of antigens or antibodies labeled with an enzyme, so that the resulting conjugates have both immunological and enzymatic activity. As on of the components (capture antibody) is coating a support (immunosorbent) the antigen antibody reaction will remain immobilized and therefore, is easily revealed by the addition of a specific substrate.
After incubation with the sample and subsequent washing, an antigen-specific HRP-conjugated antibody is added. After an intermediate wash to remove any excess antibody, a colourimetric reaction mediated by the HRP enzyme is observed with the naked eye when the substrate solution (TMB) is added. This reaction can be qualified using a spectrophotometer or colorimeter.
Recomended usage: This kit is intended for RUO in the identification and quantification of exosomes from human sera samples.
Application: ELISA
Storage Temperature: Store in the dark at 2-8 °C
Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed (at least, one year)
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB) with the plasma membrande. They are thought to provide a means of intercellular communication and of transmission of macromoleculaes between cells allowing the spread of lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.
Product Description:
ExoELISA-Step is an assay based on the use of antigens or antibodies labeled with an enzyme, so that the resulting conjugates have both immunological and enzymatic activity. As on of the components (capture antibody) is coating a support (immunosorbent) the antigen antibody reaction will remain immobilized and therefore, is easily revealed by the addition of a specific substrate.
After incubation with the sample and subsequent washing, an antigen-specific HRP-conjugated antibody is added. After an intermediate wash to remove any excess antibody, a colourimetric reaction mediated by the HRP enzyme is observed with the naked eye when the substrate solution (TMB) is added. This reaction can be qualified using a spectrophotometer or colorimeter.
Recomended usage: This kit is intended for RUO in the identification and quantification of exosomes from human sera samples.
Application: ELISA
Storage Temperature: Store in the dark at 2-8 °C
Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed (at least, one year)
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