Exosome Standard: Exosomes from PC-3, a human metastatic prostate cancer cell line
€265.00
In stock
SKU
IS-ExoPC3
Introduction
Exosomes are small (~40‐100 nm) extracellular vesicles (EVs) released from all cell types upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB) (1) with the plasma membrane. Exosomes found in body fluids and cell culture supernatants. They are though to provide a means of intercellular communication(2,3) and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment(4) and the immune response(5, 6).
PRODUCT DESCRIPTION
Lyophilized exosomes (~1x1012) derived from human cancer cell line (8,9).
Exosomes are isolated by differential ultracentrifugation (7).
• Tested application: Flow Cytometry (FMC), Nanoparticles Analysis (NTA Tracking Nanosight), Western Blot (WB), BCAProtein Assay, Mass Spectrometry, RT-qPCR.
• Species reactivity: Human
• Presentation: Lyophilized
• Reconstitution of Exosomes: For reconstitution, we recommended adding sterile, distilled water to achieve a final exosome concentration of 1μg/μl (e.g., for 100 μg standard, add 100 μl of dH2O). After the addition of water, recap vial and briefly vortex making sure that the liquid has been gently distributed and has covered the entire inside of the vial.
After vortexing, make sure that the solution is collected at the bottom of the vial, if not, centrifuge shortly the vial solution. Now the standard is ready to use
Exosomes are small (~40‐100 nm) extracellular vesicles (EVs) released from all cell types upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB) (1) with the plasma membrane. Exosomes found in body fluids and cell culture supernatants. They are though to provide a means of intercellular communication(2,3) and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment(4) and the immune response(5, 6).
PRODUCT DESCRIPTION
Lyophilized exosomes (~1x1012) derived from human cancer cell line (8,9).
Exosomes are isolated by differential ultracentrifugation (7).
• Tested application: Flow Cytometry (FMC), Nanoparticles Analysis (NTA Tracking Nanosight), Western Blot (WB), BCAProtein Assay, Mass Spectrometry, RT-qPCR.
• Species reactivity: Human
• Presentation: Lyophilized
• Reconstitution of Exosomes: For reconstitution, we recommended adding sterile, distilled water to achieve a final exosome concentration of 1μg/μl (e.g., for 100 μg standard, add 100 μl of dH2O). After the addition of water, recap vial and briefly vortex making sure that the liquid has been gently distributed and has covered the entire inside of the vial.
After vortexing, make sure that the solution is collected at the bottom of the vial, if not, centrifuge shortly the vial solution. Now the standard is ready to use
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