Exostep General Kit(CD63 Capture Beads +CD81 Biotin + STV PE) RUO

Introduction
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.

Recomended usage
This kit is intended for RUO in the purification and identification of human exosomes from Cell Culture Samples. For flow cytometric detection, >0,125 μg which corresponds with >1,5 * 10^8 vesicles form PC3 cell culture media.
Lyophilized exosomes are standards to be used during exosomes assays.

Application: Flow Cytometry

Storage Temperature: Store in the dark at 2-8 °C

Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed (at least, one year)