Human H2A (Histone H2A) ELISA Kit
€0.00
In stock
SKU
ELK4989
Catalog Number: ELK4989
Reactivity: Human
Standard Range: 0.32-20 ng/mL
Sensitivity: 0.124 ng/mL
Information
Request Manual
Questions? Contact us!
Reactivity: Human
Standard Range: 0.32-20 ng/mL
Sensitivity: 0.124 ng/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H2A(H2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone H2A(H2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H2A(H2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone H2A(H2A) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Developmental science
Sample Type:
Tissue homogenates, cell lysates and other biological fluids.
Assay Type: Sandwich
Standard: 20 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H2A(H2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone H2A(H2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone H2A(H2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone H2A(H2A) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Developmental science
Sample Type:
Tissue homogenates, cell lysates and other biological fluids.
Assay Type: Sandwich
Standard: 20 ng/mL
Assay Length: 4.5h
Assay Length: For Research Use Only
| Is Featured? | No |
|---|
Write Your Own Review