IGF-1 mouse active recombinant protein

IGF-1 mouse active recombinant protein

€295.00
In stock
SKU
600188
Catalog Number: 600188
Size: 0.05 mg
Datasheet
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Protein Family: Cytokines

Pathway and Disease: Cancers, Cell Growth and Death, Cell Communication, Nervous and Sensory Systems, Signal Transduction

Alternate Names: Insulin-like growth factor I, IGF1, Somatomedin, IGF-I, Igf-1Description:
Insulin-like growth factor I (IGF1) is a well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. IGF1 has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to Insulin-Like-Growth Factor 2 (IGF2), which is a major fetal growth factor. Recombinant Mouse IGF1 produced in E. coli is a single non-glycosylated polypeptide chain containing 70 amino acids with a MW of 7,600 Da.

Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Applications: E, WB

Application Notes:
Recombinant Mouse IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of MCF-7 cells, is less than 1.0 ng/ml.

Accession No.: P05017

MW: 7600 Da

Sequence:
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA

Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Format:
Each vial contains 50 ug of lyophilized protein. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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