L-Lactic Acid/Lactate (LA) Colorimetric Assay Kit

Detection Principle:
Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.
Instrument: Spectrophotometer
Assay Time: 60 min

Tested Sample Types: Serum,plasma,cell culture supernatant,tissue,cells

Research Area's: Glycolysis & Carbohydrates

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: http://www.ijcem.com/files/ijcem0093681.pdf