NeuN (clone ), anti-human, mouse, rat

Background:
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

Alternative Name:
RNA binding protein fox-1 homolog 3, Fox-1 homolog C, RBFOX3, NeuN, Fox-3, Fox3, Neuronal Marker

Application Dilution: WB: 1:1000-1:2000, ICC/IF: 1:50-1:200, IHC: 1:200-1:500, FC: 1:50-1:100

Specificity: This antibody detects endogenous levels of NeuN and does not cross-react with related proteins.

Immunogen:
Recombinant antibody.

MW: Predicted band size: 34KDa, Observed band size: 46, 48KDa

Swis Prot.: A6NFN3

Purification & Purity:
Protein A affinity purified

Format:
Recombinant Rabbit Monoclonal Antibody. 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.