Protein A Blue Separopore® 4B-MB
€0.00
In stock
SKU
BW-20181042
Application:
Protein A Blue-Separopore® 4B-MB functions same as conventional Protein A affinity beads, but offers enhanced visibility due to blue colored Separopore® macrobeads. The blue colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in immunoprecipitation (IP) procedures, thereby increasing efficiency and yield.
Affinity matrix with immobilized Protein-A by CNBr activation coupling method.
For immunoprecipitation and co-immunoprecipitation experiments.
Reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Recombinant Protein-A (42 kDa protein)
Matrix: Blue Separopore® 4B-MB (agarose macrobeads, 4%)
Particle size range: 150 – 265 µm
Matrix activation: Cyanogen bromide
Ligand density: ~3 mg Protein-A / ml medium
Binding capacity: ~ 20 mg human IgG / ml drained gel
Molecular weight range: 6 x 104 - 2 x 107
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Storage: 2 – 8 °C
Supplied as suspension in 0.5M NaCl, 0.02% sodium azide
References:
Extension of the selection of protein chromatography and the rate model to affinity chromatography. J Mol Recognit. (2010) 23: 609-17.
Affinity purification of proteins using expanded beds. J Chromatogr. (1992) 597: 129-45.
Two-dimensional electrophoresis of cerebrospinal fluid and ventricular fluid proteins, identification of enriched and unique proteins, and comparison with serum. J Neurochem. (1984) 43: 1277-85.
Selective binding of heat- and antigen-aggregated IgG to Blue-Sepharose. Radioimmunoassay of circulating immune complexes. J Immunol Methods. (1983) 65: 207-15.
Partial purification of organ-specific neoantigens from human colon and breast cancer by affinity chromatography with human tumour-specific gamma-globulin. Br J Cancer. (1980) 41: 86-99.
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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Protein A Blue-Separopore® 4B-MB functions same as conventional Protein A affinity beads, but offers enhanced visibility due to blue colored Separopore® macrobeads. The blue colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in immunoprecipitation (IP) procedures, thereby increasing efficiency and yield.
Affinity matrix with immobilized Protein-A by CNBr activation coupling method.
For immunoprecipitation and co-immunoprecipitation experiments.
Reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Recombinant Protein-A (42 kDa protein)
Matrix: Blue Separopore® 4B-MB (agarose macrobeads, 4%)
Particle size range: 150 – 265 µm
Matrix activation: Cyanogen bromide
Ligand density: ~3 mg Protein-A / ml medium
Binding capacity: ~ 20 mg human IgG / ml drained gel
Molecular weight range: 6 x 104 - 2 x 107
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Storage: 2 – 8 °C
Supplied as suspension in 0.5M NaCl, 0.02% sodium azide
References:
Extension of the selection of protein chromatography and the rate model to affinity chromatography. J Mol Recognit. (2010) 23: 609-17.
Affinity purification of proteins using expanded beds. J Chromatogr. (1992) 597: 129-45.
Two-dimensional electrophoresis of cerebrospinal fluid and ventricular fluid proteins, identification of enriched and unique proteins, and comparison with serum. J Neurochem. (1984) 43: 1277-85.
Selective binding of heat- and antigen-aggregated IgG to Blue-Sepharose. Radioimmunoassay of circulating immune complexes. J Immunol Methods. (1983) 65: 207-15.
Partial purification of organ-specific neoantigens from human colon and breast cancer by affinity chromatography with human tumour-specific gamma-globulin. Br J Cancer. (1980) 41: 86-99.
Related products:
Avidin-Separopore® 6B (SKU # 20181060)
Calmodulin-Separopore® 4B (SKU # 20181001)
E. coli Protein-Separopore® 4B (SKU # 20181006)
Gelatin-Separopore® 4B (SKU # 20181012)
Heparin-Separopore® 4B (SKU # 20181013)
KLH-Separopore® 4B (SKU # 20181016)
Papain-Separopore® 4B (SKU #20181027)
Protein A-Separopore® 4B-CL (SKU # 20181029)
Protein A-Separopore® 4B-CL (Lyophilized) (SKU # 20181028)
Protein A Red-Separopore® 4B-MB (Macrobeads) (SKU # 20181086)
View-IP™ Protein-A Red Separopore® 4B-CL (SKU # 20181041)
Protein G-Separopore® 4B-CL (SKU # 20181030)
Streptavidin-Separopore® 4B (SKU # 20181037)
Ubiquitin-Separopore® 4B (SKU # 20181040)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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