Protein G Red Separopore® 4B Microbeads viewIP™

Application:
The red-colored agarose bead affinity matrix has immobilized Recombinant Protein G. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant by visual monitoring of the red beads. This results in greater consistency and more accurate data due to less sample loss. View-IP™ Protein G Red-Separopore® 4B-CL is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Ligand: Native Protein G from Streptococcus sp.
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size (range): 52 – 165 µm
Matrix activation: Cyanogen bromide
Binding capacity: ~ 8 mg human IgG/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3-9 (for use), 2-10 (for cleaning)
Supplied as 50% suspension in PBS containing 50% glycerol.
Storage: 2 – 8 °C, For long term, store at -20 °C

References:
Purification of an inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase activity from rat liver and the evaluation of its substrate specificity. J Biol Chem. (1991) 266: 16499-506.
Immunological measurement of human 17 beta-hydroxysteroid dehydrogenase. J Steroid Biochem. (1990) 36: 673-80.
Purification of the thromboxane A2/prostaglandin H2 receptor from human blood platelets. J Biol Chem. (1989) 264: 16496-501.
Purification and regulatory properties of pigeon erythrocyte pyruvate kinase. Comp Biochem Physiol B. (1989) 93: 697-706.
A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju. Peptides. (2004) 25: 11-7.
Preparation of highly purified momordin II without ribonuclease activity. Life Sci. (1999) 65: 1485-91.
The heparin/heparan sulfate-binding site on apo-serum amyloid A. Implications for the therapeutic intervention of amyloidosis. J Biol Chem. (1999) 274: 7172-81.
Purification and characterization of rho-crystallin from Japanese common bullfrog lens. J Biol Chem. (1990) 265: 9914-23.

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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®, ViewIP™