SRB Cytotoxicity Assay Kit

Sulforhodamine B (SRB) Cytotoxicity Assay Kit is a sensitive, reproducible and easy-to-use assay based on the ability of SRB to bind to protein components of cells that have been fixed to tissue culture plates. SRB is a bright-pink aminoxanthene dye with two sulfonic groups that bind to basic aminoacid residues under mild acidic conditions and dissociate under basic conditions.

As the binding of SRB is stoichiometric, the amount of dye extracted from stained cells is directly proportional to the cell mass. The fixed dye is solubilized and is measured photometrically at OD 540 nm with a reference filter of 690 nm. The OD values correlate with total protein content and therefore with cell number.

Advantages & Features
- Sensitive.
- Easy-to-use.
- Fast: avoids time-sensitive measurement.
- Reproducible.
- Great linearity.
- Good signal-to-noise ratio.
- Stable end-point.

Includes
- 0.4 g SRB Dye
- 100 mL Fixative Reagent
- 100 mL Dye Wash Solution (10x)
- 200 mL SRB Solubization Buffer

Applications
- Detection of cell toxicity, death, viability or proliferation.
- High throughput screening.

Shipping & Storage
Shipped in: Ambient Temperature.
Storage: Room Temperature.
Recommendations: Protect the product from light.