0.5M EDTA (pH 8.0)

Ethylene-diamine-tetra acetic acid (EDTA) is a high quality and convenient solution that chelates a variety of polyvalent cations such as Ca2+ and Mg 2+. EDTA is usually used as an inactivator of metal-dependent enzymes, preventing damage to DNA and RNA.

In cell cultures, it is used to avoid clumping of cells in liquid suspensions, as EDTA binds to calcium and prevents joining of cadherins between cells.

GMP-grade is also available.

Advantages & Features
- Reliable: stringent quality control standards to guarantee lot-to-lot consistency.
- High Quality: free of DNAse, RNase or protease contamination.
- Time-saving due its ready-to-use format that saves experiment preparation time.
- Safe: prevents damage to DNA and RNA.

Specifications
- Chemicals: Analytical grade.
- Concentration: 0.5 M EDTA.
- pH: 8± 0.05 at 25 ºC.

Includes
Pouch format:
- Exactly pre-weighed powder (500 mL/pouch)

Aqueous solution format:
- 100 mL of 0.5M EDTA Solution

Applications
- Anticoagulant for blood samples and its storage.
- Abduct the metal required to metal-dependent enzyme, inactivating the reactions.
- Avoid junctions between cells by cadherins, usually used to cell culture procedures.
- Used in TAE and TBE buffers because it inhibits metal-dependent nucleases by chelating the divalent cations (Ca2+ Mg2+), protecting the DNA from nucleases during the run.
- Added to TE buffer, used to solubilize DNA and RNA, inactivating nucleases by binding to metals cations required by these enzymes.

Shipping & Storage
Shipped in: Ambient temperature.
Storage: Room temperature.
Recommendations: Store the product in a dry place.