14-3-3 sigma polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Description:
Rabbit IgG polyclonal antibody for 14-3-3 protein sigma (SFN) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.

Synonyms:
14 3 3 protein sigma antibody, 14-3-3 protein sigma antibody, 1433S_HUMAN antibody, Epithelial cell marker protein 1 antibody, Er antibody, HME 1 antibody, HME1 antibody, MGC143283 antibody, Mkrn3 antibody, Mme1 antibody, OTTHUMP00000004242 antibody, RP23 137L22.11 antibody, SFN antibody, SFN protein antibody, Stratifin antibody, YWHAS antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma(140-156aa KKRIIDSARSAYQEAMD), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.