17-Hydroxyprogesterone (17-OHP) test kit

Use: Used to detect the concentration of 17-hydroxyprogesterone (17-OHP) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled 17-hydroxyprogesterone (17-OHP) and purified anti-17-hydroxyprogesterone (17-OHP) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibody and biotin-labeled 17-hydroxyprogesterone (17-OHP) and non-labeled antigen (calibrator or specimen) inhibit the competitive reaction. The binding amount of antibody and biotin-labeled 17-hydroxyprogesterone (17-OHP) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled 17-hydroxyprogesterone (17-OHP), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated 17-hydroxyprogesterone (17-OHP) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated 17-hydroxyprogesterone (17-OHP)-enzyme-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme marker. As the concentration of 17-hydroxyprogesterone (17-OHP) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation, and rapidity. It has reliable detection performance for the reduction or increase of 17-hydroxyprogesterone (17-OHP) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.