2-Iminobiotin Separopore® 6B-CL
€0.00
In stock
SKU
BW-20181056
Application:
2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from (strept)avidin conjugate preparations. Interaction with avidin and streptavidin is pH dependent: forms stable complex above pH 9.5 and dissociates at pH 4.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: 2-Iminobiotin
Matrix: Separopore® 6B-CL (crosslinked agarose beads, 6%)
Particle size range: 52 – 165 µm
Matrix activation: Amino
Molecular weight range: 6 x 104 – 2 x 107
Binding capacity: 4 mg avidin / ml drained gel
pH stability: 4 – 9
Flow Specifications: 70 – 140 cm / h
Supplied as suspension in 0.5M NaCl, 0.01M sodium phosphate (pH 6.8) and 0.02% sodium azide
Appearance: White to off-white gel suspension
References:
Complexes of streptavidin-fused antigens with biotinylated antibodies targeting receptors on dendritic cell surface: a novel tool for induction of specific T-cell immune responses. Mol Biotechnol. (2012) 51: 221-32.
Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli. Biochem J. (2004) 384: 385-90.
A new europium beta-diketone chelate for ultrasensitive time-resolved fluorescence immunoassays. Anal Biochem. (2002) 311: 57-67.
Production and purification of two recombinant proteins from transgenic corn. Biotechnol Prog. (1998) 14: 149-55.
Related products:
Biotin-Separopore® 6B-CL (SKU # 20181061)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from (strept)avidin conjugate preparations. Interaction with avidin and streptavidin is pH dependent: forms stable complex above pH 9.5 and dissociates at pH 4.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: 2-Iminobiotin
Matrix: Separopore® 6B-CL (crosslinked agarose beads, 6%)
Particle size range: 52 – 165 µm
Matrix activation: Amino
Molecular weight range: 6 x 104 – 2 x 107
Binding capacity: 4 mg avidin / ml drained gel
pH stability: 4 – 9
Flow Specifications: 70 – 140 cm / h
Supplied as suspension in 0.5M NaCl, 0.01M sodium phosphate (pH 6.8) and 0.02% sodium azide
Appearance: White to off-white gel suspension
References:
Complexes of streptavidin-fused antigens with biotinylated antibodies targeting receptors on dendritic cell surface: a novel tool for induction of specific T-cell immune responses. Mol Biotechnol. (2012) 51: 221-32.
Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli. Biochem J. (2004) 384: 385-90.
A new europium beta-diketone chelate for ultrasensitive time-resolved fluorescence immunoassays. Anal Biochem. (2002) 311: 57-67.
Production and purification of two recombinant proteins from transgenic corn. Biotechnol Prog. (1998) 14: 149-55.
Related products:
Biotin-Separopore® 6B-CL (SKU # 20181061)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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