2X Direct Blood PCR Master Mix-GC

2X Direct Blood PCR Mater Mix contains all the reagents needed for quick amplification of genomic DNA from whole blood and other body fluids. This master mix can be also applied to direct amplification of exogenous gene target, such as virus, bacteria and parasites from blood. The mix is optimized with our unique inhibitor-resistant DNA polymerase for best performance. In most case, you can directly add sample into PCR master mix without pre-lysis.

There is the choice of an inert green dye in the mix, used for direct gel loading. The PCR product can be directly loaded on agarose gel without addition of loading dye for electrophoresis.

GC version works for GC-rich and complex target.

Highlight

Time saving: No need for DNA extraction or pre-lysis of sample as other similar commercial kits
Convenient and flexibility: Only need adding primers and sample in 2X mix. The similar kit in which the enzyme is packed separately is available as well (Click here), so you can titrate enzyme to find the best concentration for the target
High inhibitor-resistance: Can amplify target in PCR containing high concentration of PCR inhibitors
Specificity: GC version works for >65% GC content and complex target, increases specificity
Simply analysis: Directly loading products for electrophoresis after PCR

Applications

Genotyping
Mutation detection
Transgene detection
Knockout analysis
Sequencing
Pathogen detection
Forensic identification