2X Direct Cell PCR Master Mix

2X Direct Cell PCR Master Mix contains all the reagents needed for quick amplification of genomic DNA from cells. This master mix can be also applied to direct amplification of exogenous target genes, such as virus, bacteria and parasites from cells / cell culture. The mix is optimized with our unique inhibitor-resistant DNA polymerases and buffer for best performance. There is the choice of an inert green dye in the mix, used for direct gel loading. The PCR product can be directly loaded onto agarose gel without addition of loading dye for electrophoresis.

GC version works for GC-rich and complex target.

Highlight

Time saving: No need for DNA extraction or pre-lysis of samples
Convenient and flexibility: Only need adding primers and sample in 2X mix. The similar kit in which the enzyme is packed separately is available as well (Click here), so you can titrate enzyme to find the best concentration for the target
High inhibitor-resistance: Resistance to inhibitors in both cells and media
Specificity: GC version works for >65% GC content target, increases specificity
Simply analysis: Directly loading products for electrophoresis

Applications

Genotyping
Colony screening
Mutation detection
Pathogen detection