4E-BP1 (Phospho-Thr45) polyclonal, anti-human, mouse, rat

Specificity:
4E-BP1 (Phospho-Thr45) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr45.

Synonyms: 4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I

Immunogen:
The antiserum was produced against synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr45.

Immunogen Sequence:
13-62

Target Modification: Phospho

Modification Sites: H:T46 M:T45 R:T45

MW (kDa): 12 kDa

NCBI Gene Symbol: EIF4EBP1

Database Links:
Gene ID: 1978
Swiss Prot No.: Q13541
OMIM No.: 602223
Unigene No.: Hs.411641

Application Dilutions: WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:20000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.