5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit
Catalog Number: ACE-E0089
Size: 96 tests/kit
Detection Range: 1.56—100 ng/mL
Sensitivity: 0.94 ng/mL
Datasheet
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Size: 96 tests/kit
Detection Range: 1.56—100 ng/mL
Sensitivity: 0.94 ng/mL
Datasheet
Request Information
Price on request
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You are enquiring about 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit
Description:
This ELISA kit applies to the in vitro quantitative determination of 5-HIAA concentrations in serum, plasma and other biological fluids.
Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 5-HIAA. During the reaction, 5-HIAA in the sample or standard competes with a fixed amount of 5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-HIAA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 5-HIAA in the samples is then determined by comparing the OD of the samples to the standard curve.
Species reactivity:
Universal
Sample Type: Serum, plasma and other biological fluids
Storage instruction: -20˚C and 4˚C for 6 months
This ELISA kit applies to the in vitro quantitative determination of 5-HIAA concentrations in serum, plasma and other biological fluids.
Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 5-HIAA. During the reaction, 5-HIAA in the sample or standard competes with a fixed amount of 5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-HIAA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 5-HIAA in the samples is then determined by comparing the OD of the samples to the standard curve.
Species reactivity:
Universal
Sample Type: Serum, plasma and other biological fluids
Storage instruction: -20˚C and 4˚C for 6 months
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