6X BX/ Loading buffer

6X BX/Loading Buffer is used as a loading dye for visual tracking of DNA migration during electrophoresis. It incorporates Bromophenol blue and Xylene Cyanol FF as tracking dye. Bromophenol blue migrates fast in the agarose gel and corresponds to the migration of a 300 – 500 bp long DNA fragment in a 1% agarose gel. Xylene cyanol FF migrates comparatively slower and corresponds to the migration of a 4,000 – 5,000 bp long DNA fragment in a 1% agarose gel.

EDTA is included in the solution to protect the sample from nuclease degradation. Glycerol is added to provide high density to the solution. Due to its high density, the sample settles at the bottom of the well. It also helps DNA samples to be confined in the well without diffusing out from it.

Advantages & Features
- High quality.
- Proven performance for DNA Ladders preparation.
- Optimized to protect samples from nuclease degradation, provide high density and for sample confinement in the well without diffusing out from it.

Specifications
Concentration: 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% Glycerol, 10 mM Tris-HCl (pH 7.6) and 60 mM EDTA.

Includes
5 x 1 mL of selected Loading Buffer

Applications
Preparation of DNA Ladders, Markers and Samples for loading on agarose or polyacrylamide gels.

Shipping & Storage
Shipped in: Gel pack.
Storage: 4 °C.