6X OX/Loading Buffer

6X OX/Loading Buffer is used as a loading dye for visual tracking of DNA migration during electrophoresis. It incorporates Orange G and Xylene Cyanol FF as tracking dye. Orange G migrates comparatively very fast and corresponds to the migration of a 50 bp long DNA fragment in 1% agarose gel. Xylene cyanol FF migrates comparatively slower and corresponds to the migration of a 4,000 – 5,000 bp long DNA fragment in a 1% agarose gel.

The EDTA is included in the solution to protect samples from nuclease degradation. Glycerol is added to provide high density to the solution. Due to its high density, sample settles at the bottom of the well. It also helps DNA samples to be confined in the well without diffusing out from it.

Advantages & Features
- High quality.
- Proven performance for DNA Ladders preparation.
- Optimized to protect samples from nuclease degradation, provide high density and for sample confinement in the well without diffusing out from it.


Specifications
Concentration: 0.15% orange G, 0.03% xylene cyanol FF, 60% Glycerol, 10 mM Tris-HCl (pH 7.6) and 60 mM EDTA.

Includes
5 x 1 mL of selected Loading Buffer

Applications
Preparation of DNA Ladders, Markers and Samples for loading on agarose or polyacrylamide gels.

Shipping & Storage
Shipped in: Gel pack.
Storage: 4 °C.