7-AAD solution

Description:
The 7-AAD (7-amino-actinomycin D) solution can be used to exclude non-viable cells in flow cytometry assays. It is excited by 488 laser light and is detected by the far red 650nm long-pass filter. Unlike propidium iodide, 7-AAD has minimal spectral overlap with the fluorescence emissions of PE and FITC.

References:
O'Brien, M. C., & Bolton, W. E. (1995). Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. Cytometry, 19(3), 243-255.
Schmid, I., Krall, W. J., Uittenbogaart, C. H., Braun, J., & Giorgi, J. V. (1992). Dead cell discrimination with 7‐amino‐actinomcin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry, 13(2), 204-208.