α-Lactose Separopore® 6B-CL (Divinyl Sulfone-Coupled)
€0.00
In stock
SKU
BW-20181174
Application: α-Lactose-Separopore® 6B-CL is used in protein chromatography, affinity chromatography and carbohydrate matrices. α-Lactose-Separopore® 6B-CL has been used to study microneme proteins and host-parasite cell surface receptors. Lactose-Separopore® 6B-CL may be used to isolate lactose binding proteins, such as lectins and glycoproteins.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Lactose Matrix: Separopore® 6B-CL (crosslinked agarose beads, 6%)
Particle size range: 52 – 165 µm.
Matrix activation: Divinyl Sulfone
Matrix attachment: Hydroxyl
Matrix spacer: 5 atoms
Binding capacity: 20 – 25 mg lectin from Arachis hypogaea / ml drained gel
Storage: 2 – 8 °C.
Suspension in 0.15M NaCl, 0.01M sodium phosphate, pH 6.8, containing 0.02% sodium azide
References:
Isolation of galectin-1 from human platelets: its interaction with actin. Protein J. (2012) 31: 8-14.
Purification and molecular cloning of a new galactose-specific lectin from Bauhinia variegata seeds. J Biosci. (2008) 33: 355-63.
Characterization and purification of the discoidin domain-containing protein retinoschisin and its interaction with galactose. Biochemistry. (2008) 47: 9098-106.
Galectin-3 expression in human papillary thyroid carcinoma. Cancer Genomics Proteomics. (2008) 5: 117-22.
Vaccination with microneme protein NcMIC4 increases mortality in mice inoculated with Neospora caninum. J Parasitol. (2007) 93: 1046-55.
The second glutamic acid in the C-terminal CRD affects the carbohydrate-binding properties of recombinant galectins of Haemonchus contortus. Vet Parasitol. (2007) 148: 247-55.
Amino acid sequence and characterization of C-type lectin purified from the snake venom of Crotalus ruber. Comp Biochem Physiol B Biochem Mol Biol. (2007) 146: 299-306.
Unexpected different binding of mistletoe lectins from plant extracts to immobilized lactose and N-acetylgalactosamine. Anal Chem Insights. (2007) 2: 43-50.
Anti-glycosyl antibodies in lipid rafts of the enterocyte brush border: a possible host defense against pathogens. Am J Physiol Gastrointest Liver Physiol. (2005) 289: G1100-7.
Identification and characterization of a Neospora caninum microneme-associated protein (NcMIC4) that exhibits unique lactose-binding properties. Infect Immun. (2004) 72: 4791-800.
Isolation and characterization of galectins in the mammalian retina. Invest Ophthalmol Vis Sci. (2001) 42: 2164-72.
Cloning and characterization of a beta-galactoside-binding protein (galectin) from the gut of the gastrointestinal nematode parasite Haemonchus contortus. Parasitology. (1999) 119: 483-90.
Processing of preproricin in transgenic tobacco. Protein Expr Purif. (1999) 15: 188-95.
Actinomyces serovar WVA963 coaggregation-defective mutant strain PK2407 secretes lactose-sensitive adhesion that binds to coaggregation partner Streptococcus oralis 34. Oral Microbiol Immunol. (1998) 13: 337-40.
Murine microglial cells express functionally active galectin-3 in vitro. J Neurosci Res. (1998) 51: 49-57.
Identification of a 95 kDa putative adhesin from Actinomyces serovar WVA963 strain PK1259 that is distinct from type 2 fimbrial subunits. Microbiology. (1997) 143: 835-46.
Identification of galectin-3 as a factor in pre-mRNA splicing. Proc Natl Acad Sci U S A. (1995) 92: 1213-7.
Purification and characterization of the N-terminal domain of galectin-4 from rat small intestine. FEBS Lett. (1995) 359: 169-72.
Characterization of isolectins in Tetracarpidium conophorum seeds (Nigerian walnut). Glycoconj J. (1994) 11: 299-303.
Purification and properties of a novel beta-galactosidase or exo-(1-->4)-beta-D-galactanase from the cotyledons of germinated Lupinus angustifolius L. seeds. Planta. (1994) 192: 502-11.
ATP-dependent translocation of ricin across the membrane of purified endosomes. J Biol Chem. (1993) 268: 23661-9.
Peanut lectin: a mitogen for normal human colonic epithelium and human HT29 colorectal cancer cells. J Natl Cancer Inst. (1992) 84: 1410-6.
Related products:
Dextran-Separopore® 4B-CL (SKU # 20181005)
Fucose-Separopore® 4B-CL (SKU # 20181010)
Galactose-Separopore® 4B-CL (SKU # 20181011)
Glucose-Separopore® 4B-CL (SKU # 20181076)
Maltose-Separopore® 4B (SKU # 20181072)
Mannose-Separopore® 4B-CL (SKU # 20181019)
Guar Gum-Separopore® 4B-CL (SKU # 20181052)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-Hazardous
Storage: 2-8°C
Appearance Form: Liquid (Suspension)
Brand Name: bioPLUS™, Separopore®
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Lactose Matrix: Separopore® 6B-CL (crosslinked agarose beads, 6%)
Particle size range: 52 – 165 µm.
Matrix activation: Divinyl Sulfone
Matrix attachment: Hydroxyl
Matrix spacer: 5 atoms
Binding capacity: 20 – 25 mg lectin from Arachis hypogaea / ml drained gel
Storage: 2 – 8 °C.
Suspension in 0.15M NaCl, 0.01M sodium phosphate, pH 6.8, containing 0.02% sodium azide
References:
Isolation of galectin-1 from human platelets: its interaction with actin. Protein J. (2012) 31: 8-14.
Purification and molecular cloning of a new galactose-specific lectin from Bauhinia variegata seeds. J Biosci. (2008) 33: 355-63.
Characterization and purification of the discoidin domain-containing protein retinoschisin and its interaction with galactose. Biochemistry. (2008) 47: 9098-106.
Galectin-3 expression in human papillary thyroid carcinoma. Cancer Genomics Proteomics. (2008) 5: 117-22.
Vaccination with microneme protein NcMIC4 increases mortality in mice inoculated with Neospora caninum. J Parasitol. (2007) 93: 1046-55.
The second glutamic acid in the C-terminal CRD affects the carbohydrate-binding properties of recombinant galectins of Haemonchus contortus. Vet Parasitol. (2007) 148: 247-55.
Amino acid sequence and characterization of C-type lectin purified from the snake venom of Crotalus ruber. Comp Biochem Physiol B Biochem Mol Biol. (2007) 146: 299-306.
Unexpected different binding of mistletoe lectins from plant extracts to immobilized lactose and N-acetylgalactosamine. Anal Chem Insights. (2007) 2: 43-50.
Anti-glycosyl antibodies in lipid rafts of the enterocyte brush border: a possible host defense against pathogens. Am J Physiol Gastrointest Liver Physiol. (2005) 289: G1100-7.
Identification and characterization of a Neospora caninum microneme-associated protein (NcMIC4) that exhibits unique lactose-binding properties. Infect Immun. (2004) 72: 4791-800.
Isolation and characterization of galectins in the mammalian retina. Invest Ophthalmol Vis Sci. (2001) 42: 2164-72.
Cloning and characterization of a beta-galactoside-binding protein (galectin) from the gut of the gastrointestinal nematode parasite Haemonchus contortus. Parasitology. (1999) 119: 483-90.
Processing of preproricin in transgenic tobacco. Protein Expr Purif. (1999) 15: 188-95.
Actinomyces serovar WVA963 coaggregation-defective mutant strain PK2407 secretes lactose-sensitive adhesion that binds to coaggregation partner Streptococcus oralis 34. Oral Microbiol Immunol. (1998) 13: 337-40.
Murine microglial cells express functionally active galectin-3 in vitro. J Neurosci Res. (1998) 51: 49-57.
Identification of a 95 kDa putative adhesin from Actinomyces serovar WVA963 strain PK1259 that is distinct from type 2 fimbrial subunits. Microbiology. (1997) 143: 835-46.
Identification of galectin-3 as a factor in pre-mRNA splicing. Proc Natl Acad Sci U S A. (1995) 92: 1213-7.
Purification and characterization of the N-terminal domain of galectin-4 from rat small intestine. FEBS Lett. (1995) 359: 169-72.
Characterization of isolectins in Tetracarpidium conophorum seeds (Nigerian walnut). Glycoconj J. (1994) 11: 299-303.
Purification and properties of a novel beta-galactosidase or exo-(1-->4)-beta-D-galactanase from the cotyledons of germinated Lupinus angustifolius L. seeds. Planta. (1994) 192: 502-11.
ATP-dependent translocation of ricin across the membrane of purified endosomes. J Biol Chem. (1993) 268: 23661-9.
Peanut lectin: a mitogen for normal human colonic epithelium and human HT29 colorectal cancer cells. J Natl Cancer Inst. (1992) 84: 1410-6.
Related products:
Dextran-Separopore® 4B-CL (SKU # 20181005)
Fucose-Separopore® 4B-CL (SKU # 20181010)
Galactose-Separopore® 4B-CL (SKU # 20181011)
Glucose-Separopore® 4B-CL (SKU # 20181076)
Maltose-Separopore® 4B (SKU # 20181072)
Mannose-Separopore® 4B-CL (SKU # 20181019)
Guar Gum-Separopore® 4B-CL (SKU # 20181052)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-Hazardous
Storage: 2-8°C
Appearance Form: Liquid (Suspension)
Brand Name: bioPLUS™, Separopore®
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