A-RAF (Phospho-Tyr302) polyclonal, anti-human, mouse, rat

Specificity:
A-RAF (Phospho-Tyr302) Antibody detects endogenous levels of A-RAF only when phosphorylated at Tyr302.

Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2

Immunogen:
The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302.

Immunogen Sequence:
276-325

Target Modification: Phospho

Modification Sites: H:Y302 M:Y300 R:Y300

MW (kDa): 67 kDa

NCBI Gene Symbol: ARAF

Database Links:
Gene ID: 369
Swiss Prot No.: P10398
OMIM No.: 311010
Unigene No.: Hs.225936/Hs.446641

Application Dilutions: WB: 1:500~1:1000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:20000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.