A/Tn/GalNAca1 Antigen (clone BRIC 66) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human A/Tn/GalNAcalpha 1 Antigen Affinity purified. The histo-blood group A antigen is defined by the carbohydrate structure at the non-reducing termini of oligosaccharide chains of glycoproteins and glycolipids. The minimal determinant struc­ture of the A antigen is GalNAc(a1-3) Fuc(a1-2) Gal (beta1-R). Termi­nal GalNac(a-1) also occurs in other linkages. The A antigen is widely distributed in the erythrocytes, cells and tissues, and in body fluids of A positive individuals, and also in other animals. The reactive group GalNAc(a1-Ser) (or Thr) is exposed in sialoglycoproteins of blood cells of individu­als with the Tn syndrome, irrespective of their A antigen status.

Synonyms: A Antigen, GalNAc(a1-3) Fuc(a1-2) Gal (beta1-R), Terminal GalNac(a-1), Sialoglycoprotein

Clone: BRIC 66

Isotype: Mouse IgM kappa light chain

Immunogen: BRIC 66 was made in response to immunisation with A active ovar­ian cyst glycoprotein.

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: Immunofluorescence, Immunoblotting, Immunocytochemical techniques.

Working Dilution: Optimal dilution should be performed by user

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Clausen, H., Hakomori, S., Vox Sang. 56: 1-20 (1989)
2. Oriol, R., Le Pendu, J., Mollicone, R., Vox Sang. 51: 161-171 (1986)
3. King, M.J., Parsons, S.F., Wu, A.M., Jones, N. Transfusion 31: 142-149 (1991)
4. Brooks S.A et al (1995) Expression of alph-GalNAc glycoproteins by breast cancers. Bristish J. of Cancer 71, 1033-1038.