Acetylated Lysine polyclonal, anti-human, mouse, rat

Protein Family: Enzymes

Pathway and Disease: Signaling Molecules and Interaction, Transcription

Description:
DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds DNA by acetylation of lysine residues of histones. Research has shown that acetylation of non-histone proteins (e.g. transcription factors) and histones are involved in transcription. Histone acetyltransferases (HAT) acetylate the conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) on lysine residues, whereas histone deacetylases (HDAC) remove them. This pan-specific antibody recognizes proteins with acetyllysine residues. This antibody has been utilized for proteomic studies of protein acetylation, immunoaffinity chromatography separation and isolation of acetylated proteins and peptides from protease-digested whole cells.

Alternate Names: Acetylated Lysine, AcK, Acetyllysine, Acetyl-lysine, AcLys

Application Notes: Cat. No. 251115 has been tested on acetylated histones, acetylated BSA and acetylated MBP. There is no reaction with non-acetylated proteins. E: 1:500-1:2,000; WB: 1:200-1:1000; IF: 1:50-1:100; IHC: 1:100-1:500

Antigen: Acetylated KLH-conjugates

Format: Each vial contains 0.1 mg IgG in 0.4 ml (0.25 mg/ml) of PBS pH7.4, 50% glycerol. Antibody was purified by using acetyllysine-affinity chromatography.

Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.