Acid Phosphatase (ACP) Activity Assay Kit

Detection Principle:
Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
Instrument: Microplate reader
Assay Time: 45 min

Tested Sample Types: Serum,plasma,hydrothorax,urine,cells,cell culture supernatant,animal tissue

Research Area's: Enzymes,Others

Shelf Life: 3 Months

Storage:
2-8℃