ACOT12 (R315) polyclonal, anti-human, mouse

Background:
Abstract Acetyl-CoA plays a fundamental role in cell signaling and metabolic pathways, with its cellular levels tightly controlled through reciprocal regulation of enzymes that mediate its synthesis and catabolism. ACOT12, the primary acetyl-CoA thioesterase in the liver of human, mouse, and rat, is responsible for cleavage of the thioester bond within acetyl-CoA, producing acetate and coenzyme A for a range of cellular processes. The enzyme is regulated by ADP and ATP, which is believed to be mediated through the ligand-induced oligomerization of the thioesterase domains, whereby ATP induces active dimers and tetramers, whereas apo- and ADP-bound ACOT12 are monomeric and inactive.

Alternative Name:
Acyl-coenzyme A thioesterase 12, Acyl-CoA thioesterase 12, Acyl-CoA thioester hydrolase 12, Cytoplasmic acetyl-CoA hydrolase 1, CACH-1, hCACH-1, START domain-containing protein 15, StARD15, ACOT12, CACH, CACH1, STARD15

Application Dilution:
WB: 1:500~1:1000

Specificity: ACOT12 (R315) polyclonal antibody detects endogenous levels of ACOT12 protein.

Immunogen:
Synthetic peptide, corresponding to amino acids 285-345 of Human ACOT12.

MW: ~ 62 kDa

Swiss-Prot: Q8WYK0

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.