ACTH (clone 2F6), anti-human, mouse, rat

Source:
A BALB/c mouse was immunized with ACTH synthetic peptide (aa 1-24) conjugated to KLH.Fusion partner SP2/0.

Specifications:
ACTH (same as corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. 2F6 is specific to synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH). ACTH is also produced by cells of the immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress.

Species reactivity:
Positive: human, mouse, rat.

Applications:
2 F6 is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell lung carcinomas) causing paraneoplastic syndromes by secreting ACTH.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: Pituitary gland, pituitary tumor.

References:
- Kimitsuki K. et al.J Vet Med Sci76(1) 133-138 (2014)