Actin & Tubulin Antibody Sampler Kit

Background:
Microtubules (MTs) and actin filaments are cytoskeletal elements that play an essential role in cell division, cytoplasmic organization, cell morphology, and motility. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. Actin filaments are polymers formed by one of six different actin isoforms found in vertebrates. Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. Phosphorylation may regulate the activity tubulin and actin isoforms. Cdk1 phosphorylation of Ser-172 in β-tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules. In Dictyostelium, actin phosphorylation at Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

Background References
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
Jungbluth, A. et al. (1995) FEBS Let. 375:87.
Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Baba, T. et al. (2003) J. Immunol. 170: 3762.
Winder, S.J. et al. (2005) J. Cell Sci. 118:651.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694.

Buffer/Storage:
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

Product References:
AM2021 Muirhead, G et al. (2014) J Mol Neurosci. 53(1):125 WB: rat brain
AM2021 Pritchard, AJ et al. (2014) PLoS One. 9(6):e99444 WB: mouse splenocytes
TM1541 Wang, Q. et al. (2014) J Virol. 88(9):4853 WB: HeLa
AM2021 Dutta, P et al. (2014) J Neurochem. 130(3):360 WB: rat brain
AP1671 Vonach, C. et al. (2011) British J Cancer. 105:263. WB: human endothelial cells
TM1541 Meacham, CE. et al. (2011) Nature Genetics. 41:1133. WB: mouse lymphoma
TM1541 Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. WB: U3&3MG
TM1541 Campbell, S. et al. (2010) J Biol Chem. 285:4695. WB: human HeLa cells