Adalimumab(Humira®) Pharmacokinetic ELISA
€780.00
In stock
SKU
EL-1611-011
INTRODUCTION
Adalimumab (Humira®) is a human monoclonal antibody used to block the action of tumor necrosis factor alpha (TNF-α). TNF-α is a strong proinflammatory cytokine involved in normal immunity and also various autoimmune diseases.
The Adalimumab ELISA kit is designed to measure free Adalimumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Adalimumab molecules in biological matrices.
PRINCIPLE OF THE ASSAY
This assay employs the sandwich enzyme immunoassay technique. Anti- Adalimumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Adalimumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Adalimumab present in test samples.
Product Properties
Detection method: Peroxidase / OD450
Storage and stability: Stable at -20°C for 1 year
Safety Warnings and Precautions: Research Use Only. Follow instructions.
Precision : Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
Detection limit : 1.5ng/ml
Each kit includes:
- Coated microtiter plate, 96 wells
- Calibrator diluent. - 1.8ml
- Calibrator (250 ug/mL) - 12ul
- 10X wash buffer - 25ml
- Assay buffer - 50ml
- 1000X detection reagent - 17ul
- TMB - 12ml
- TMB stop solution - 12ml
- Plate sealers - 3
Adalimumab (Humira®) is a human monoclonal antibody used to block the action of tumor necrosis factor alpha (TNF-α). TNF-α is a strong proinflammatory cytokine involved in normal immunity and also various autoimmune diseases.
The Adalimumab ELISA kit is designed to measure free Adalimumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Adalimumab molecules in biological matrices.
PRINCIPLE OF THE ASSAY
This assay employs the sandwich enzyme immunoassay technique. Anti- Adalimumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Adalimumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Adalimumab present in test samples.
Product Properties
Detection method: Peroxidase / OD450
Storage and stability: Stable at -20°C for 1 year
Safety Warnings and Precautions: Research Use Only. Follow instructions.
Precision : Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
Detection limit : 1.5ng/ml
Each kit includes:
- Coated microtiter plate, 96 wells
- Calibrator diluent. - 1.8ml
- Calibrator (250 ug/mL) - 12ul
- 10X wash buffer - 25ml
- Assay buffer - 50ml
- 1000X detection reagent - 17ul
- TMB - 12ml
- TMB stop solution - 12ml
- Plate sealers - 3
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