Adenosine Deaminase (ADA) Activity Assay Kit

Detection Principle:
Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA.
Instrument: Microplate reader
Assay Time: 40 min

Tested Sample Types: serum, plasma, animal tissue

Research Area's: Enzymes,Liver Biomarkers

Shelf Life: 6 Months

Storage:
2-8℃