AFP (clone C2), anti-human, mouse

Source:
A BALB/c mouse was immunized with alpha fetoprotein isolated from serum of hepatoma patients.
Fusion partner: SP2/0.

Specifications:
C2 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nustad to group E of a cluster of 6 major epitopes of human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa. It is expressed in fetal liver and is normally absent in health adult tissues. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.

Species reactivity:
Positive: human, mouse. Negative: cow, dog, rat.

Applications:
C2 is excellent for IHC. In paraffin sections a citrate antigen retrieval step is advised. It can also be used in ELISA, the TD-2 workshop found C2 suitable for an AFP tumor marker test, both as solid phase as well as tracer antibody in combination with any antibody of epitope groups A, B, C, or D.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: Hep-G2 cells, fetal liver or hepatocellular carcinoma.

References:
- Tsung K., et al. J. Immunol. Methods39: 363-368 (1980).
- Michell B.et al. Eur. J. Cancer Clin. Oncol.19:1239-1246 (1983).
- Yazova A.K. et al. Immunol. Lett. 25: 325-330 (1990).
- Nustad K. Et al. Tumor Biol19: 293 -300 (1998).
- Yakimenko E.F. et al. Tumor Biol19: 301-309 (1998).