AFP (clone D10), anti-human

Source:
A BALB/c mouse was immunized with alpha fetoprotein isolated from serum of hepatoma patients.
Fusion partner: Sp2/0.

Specifications:
D10 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nustad to group D of a cluster of 6 major epitopes of human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa. It is expressed in fetal liver and is normally absent in healthy adult tissues. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.

Species reactivity:
Positive: human. Negative: cow, dog, mouse, rat.

Applications:
D10 can be used for ELISA, the TD-2 workshop found D10 suitable for an AFP tumor marker test, both as solid phase as well as tracer antibody in combination with antibodies of epitope groups A and B. D10 is not suitable for IHC in paraffin sections, an appropriate antigen retrieval step has not been established.ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
RIM: +

Positive control: Hep-G2 cells, fetal liver or hepatocellular carcinoma.

References:
- Tsung K., et al. J. Immunol. Methods39: 363-368(1980).
- Michell B.et al. Eur. J. Cancer Clin. Oncol.19:1239-1246 (1983).
- YazovaA.K. et al. Immunol. Lett. 25: 325-330 (1990).
- Nustad K. Et al. Tumor Biol19: 293 -300 (1998).
- Yakimenko E.F. et al. Tumor Biol19: 301-309 (1998).