AFP (clone MBS-12), anti-human

Source:
A BALB/c mouse was immunized with alpha fetoprotein isolated from serum of hepatoma patients.
Fusion partner: NS1.

Specifications:
MBS-12 specifically detects AFP. This protein is one of the major serum proteins in the early life of mammals and through to be fetal counterpart of albumin. AFP production is reactivated in the adult during liver regeneration and hepatocarcinogenesis, though in some individuals it persists into adulthood naturally. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.

Species reactivity:
Positive: human.

Applications:
MBS-12 is excellent for IHC. In paraffin sections a citrate antigen retrieval step is required. It can also be used in flow cytometry and ELISA.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: Hep-G2 cells, fetal liver or hepatocellular carcinoma.

References:
- Stefanova, I. et al, J. Immunol. Methods.111: 67-73(1988).
- Lafuste, P, et al,Placenta23: 600-612 (2002).