Agrin polyclonal, anti-human, mouse, rat

Protein Family: CAM Ligands, Glycan Binding Proteins

Pathway and Disease: Signaling Molecules and Interaction, Nervous and Sensory Systems

Description:
Agrin is an essential extracellular matrix component that causes clustering of nicotinic acetylcholine receptors (nAChRs) and acetylcholine-esterase on the surface of muscle fibers of the neuromuscular junction. Agrin, MuSK and rapsyn are all essential components for AChR aggregation. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites increasing the predicted MW from 200 kDa to 600 kDa.

Alternate Names: Agrin, AGRN

Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500

Accession No.: A2ASQ1

Antigen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of mouse agrin.

Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 25% glycerol, 1 mg/ml BSA with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.