αII-Spectrin (a.a. 1171-1176), cleavage-specific polyclonal, anti-human ,mouse, rat

Background:
Spectrins are central components of the cytoskeleton that form a scaffold below the plasma membrane. Spectrins contain two subunits, α and β, which intertwine to form heterodimers that can self associate into elongated tetramers. α-spectrin I and β-spectrin I form heterodimers in red blood cells, while nonerythroid mammalian cells contain heterodimers of α-spectrin I and II with β-spectrin I to V. The structure of spectrins includes a succession of triple-helical repeats along with various domains, such as SH3 domain, EF hands, PH domains, and binding domains for ankyrin, actin, band 4.1, and calmodulin. α-spectrin II is a widely expressed non-erythroid spectrin that contains an SH3 domain, a calmodulin binding site, and two cleavage sites, one at Tyr-1176 for calpains and one at Asp-1185 for caspase-3. α-spectrin II and β-spectrin II, like many other spectrins, can form heterodimers that can self associate into tetramers, as well as interact with Band 4.1, F-actin, and other proteins near the plasma membrane.

Background References

Robert-Lewis, J.M. et al. (1994) J Neurosci. 14(6):3934.
Manya, H. et al. (2002) J Biol Chem. 277(38):35503.
Higuchi, M. et al. (2005) J Biol Chem. 280:15229.

Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues 1171 to 1176 in human αII-spectrin. This sequence has high homology with similar regions in rat and mouse αII-spectrin, but is not found in other spectrin family members.

Specificity: This antibody was affinity purified using αII-spectrin (a.a. 1171-1176) peptide (without carrier). The antibody detects a 136 kDa* cleaved fragment corresponding to αII-Spectrin on SDS-PAGE immunoblots of mouse diaphram treated with thapsigargin. The antibody only detects cleaved αII-Spectrin and does not detect full length αII-Spectrin.

Buffer/Storage:
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.