Akt Phospho-Regulation Antibody Sampler Kit

Background:
Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.

Buffer/Storage:
AP1001 and AM1011 are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide, and AM1141 is provided in PBS with 0.02% azide. The secondary reagents are supplied in PBS, 50% glycerol, 1 mg/ml BSA, and no azide. Store at –20°C. Stable for 1 year.

Product References:
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
AM1141 Wu, C. et al. (2012) Cell. 148(5):973. WB: mouse embryonic fibroblasts
AM1141 Paterniti, I. et al. (2011) Eur J Neurosci. 33(8):411. WB: mouse spinal cord
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
AM1011 Wei, H., Vander Heide, R. (2010) AJP Heart Circ. 298:H152. WB: rat myocardium
AM1011 Chambers, M.A. et al. (2009) J Physiol. 587:3363. WB: mouse muscle, C2C12
AM1011 Moylan, J.S. et al. (2008) AJP Cell. Phys. 295:986. WB: mouse C2C12