Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method)

Detection Principle:
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Instrument: Microplate reader
Assay Time: 80 min

Tested Sample Types: Serum,plasma,animal tissue,cells,cell culture supernatant

Research Area's: Enzymes,Liver Biomarkers

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: https://www.sciencedirect.com/science/article/abs/pii/S1043661819308473?via%3Dihub
https://www.sciencedirect.com/science/article/pii/S2213713018300014
https://works.bepress.com/dr_hesham_mustafa/26/download/
http://xueshu.baidu.com/usercenter/paper/show?paperid=1b6r0xe0kc5c0cr0t9270aq0s1431613&site=xueshu_se
https://www.ncbi.nlm.nih.gov/pubmed/31824338
https://pubmed.ncbi.nlm.nih.gov/32531799/
https://pubmed.ncbi.nlm.nih.gov/32035931/
https://iopscience.iop.org/article/10.1088/1742-6596/1374/1/012009/pdf
https://pubs.acs.org/doi/abs/10.1021/acsabm.0c00841
https://pubmed.ncbi.nlm.nih.gov/32678475/
https://pubmed.ncbi.nlm.nih.gov/32678498/
https://pubmed.ncbi.nlm.nih.gov/32705206/
https://pubmed.ncbi.nlm.nih.gov/33022138/
https://onlinelibrary.wiley.com/doi/10.1002/mnfr.202001133
https://www.sciencedirect.com/science/article/abs/pii/S1742706120307509?via%3Dihub
https://www.sciencedirect.com/science/article/abs/pii/S0142961221003434?via%3Dihub
https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.13859
https://onlinelibrary.wiley.com/doi/10.1002/mnfr.202001133