Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method)

Detection Principle:
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Instrument: Spectrophotometer
Assay Time: 100 min

Tested Sample Types: Serum,plasma,cells,animal tissue

Research Area's: Enzymes,Liver Biomarkers

Shelf Life: 6 Months

Storage:
2-8℃