ALD (Aldosterone) ELISA Kit

ALD (Aldosterone) ELISA Kit

Catalog Number: ACE-E0086
Size: 96 tests/kit
Detection Range: 31.25—2000 pg/mL
Sensitivity: 18.75 pg/mL
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Description:
This ELISA kit applies to the in vitro quantitative determination of  ALD concentrations in serum, plasma and other biological fluids.

Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ALD. During the reaction,  ALD in the sample or standard competes with a fixed amount of ALD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  ALD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  ALD in the samples is then determined by comparing the OD of the samples to the standard curve.

Species reactivity:
Universal

Sample Type: Serum, plasma and other biological fluids

Storage instruction: -20˚C and 4˚C for 6 months
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