Alkaline Phosphatase (ALP) Activity Assay Kit

Detection Principle:
Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.
Instrument: Microplate reader
Assay Time: 30 min

Tested Sample Types: Serum,plasma,tissue,cells

Research Area's: Enzymes,Liver Biomarkers

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: https://www.sciencedirect.com/science/article/pii/S2213713018300014
https://doi.org/10.3389/fphar.2021.756924
https://onlinelibrary.wiley.com/doi/10.1002/mnfr.202001133