ALKBH4 polyclonal, anti-human, mouse
€388.00
In stock
SKU
BS70932
Background:
ALKBH4 (alkB, alkylation repair homolog 4), also known as ABH4, is a 302 amino acid protein belonging to the alkB family. ALKBH4 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. ALKBH4 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome.
Alternative Name:
ABH4,
Application Dilution: WB: 1:500 - 1:2000, IF: 1:50 - 1:200
Specificity: ALKBH4 polyclonal antibody detects endogenous levels of ALKBH4 protein.
Immunogen:
Recombinant protein of human ALKBH4.
MW: ~ 34 kDa
Swis Prot.: Q9NXW9
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
ALKBH4 (alkB, alkylation repair homolog 4), also known as ABH4, is a 302 amino acid protein belonging to the alkB family. ALKBH4 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. ALKBH4 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome.
Alternative Name:
ABH4,
Application Dilution: WB: 1:500 - 1:2000, IF: 1:50 - 1:200
Specificity: ALKBH4 polyclonal antibody detects endogenous levels of ALKBH4 protein.
Immunogen:
Recombinant protein of human ALKBH4.
MW: ~ 34 kDa
Swis Prot.: Q9NXW9
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
| Is Featured? | No |
|---|
Write Your Own Review