Alpha-4 Integrin polyclonal, anti-human, mouse, rat

Protein Family: Cell Adhesion Molecules, Cellular Antigens, CAM Ligands

Pathway and Disease: Signaling Molecules and Interaction, Immune System, Infectious Diseases, Cell Motility, Cell Communication

Description:
Integrin alpha-4 (CD49d) is a receptor for fibronectin. Integrin alpha-4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-4 is also a receptor for VCAM1, recognizing the sequence Q-I-D-S in VCAM1, and a receptor for MADCAM1, recognizing the sequence L-D-T in MADCAM1. On activated endothelial cells, integrin alpha-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines, and also participates in cytolytic T-cell interactions with target cells.

Alternate Names: Integrin alpha-4, Integrin alpha-IV, VLA-4 subunit alpha, CD49 antigen-like family member D, CD49d, ITGA4, CD49D

Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:200; ICC: 1:50-1:100, in acetone fixed cells, antigen retrieval with pepsin and trypsin is recommended.

Accession No.: P13612

Antigen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human integrin alpha-4.

Format: Each vial contains 0.1 mg IgG in 0.5 ml (0.2 mg/ml) of PBS pH7.4, 50% glycerol. Antibody was purified by immunogen-affinity chromatography.

Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.

Product Citations:
Prisco AR et al. 2014. Am J Physiol Heart Circ Physiol. PMID#25539711.