alpha Tubulin Polyclonal Antibody

Background:
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Research Area: Cancer, Signal Transduction

Synonyms:
Alpha tubulin 3, Alpha-tubulin 3, B alpha 1, FLJ25113, LIS3, TBA1A, TUBA1A, TUBA3, Tubulin alpha 1a, Tubulin alpha 1A chain, Tubulin alpha 3, Tubulin alpha 3 chain, Tubulin alpha brain specific, Tubulin alpha-1A chain, Tubulin alpha-3 chain, Tubulin B alpha 1, Tubulin B-alpha-1

Immunogen: Synthesized peptide derived from the C-terminal region of human Tubulin α

Swissprot: Q71U36,P68363

Calculated MW: 50 kDa
Observed MW: 50 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.