AML1 (Phospho-Ser435) polyclonal, anti-human, mouse, rat

Specificity:
AML1 (Phospho-Ser435) Antibody detects endogenous levels of RUNX1 only when phosphorylated at Ser435.

Synonyms: Acute myeloid leukemia 1 protein, CBF-alpha 2, CBFA2, Core-binding factor, alpha 2 subunit, Oncogene AML-1, PEA2-alpha B, PEBP2-alpha B, Polyomavirus enhancer binding protein 2 alpha B subunit, RUN1, Runt-related transcription factor 1, RUNX1, SL3-3 enhan

Immunogen:
The antiserum was produced against synthesized phospho-peptide derived from human AML1 around the phosphorylation site of Ser435.

Immunogen Sequence:
Phospho

Modification Sites: H:S435, M:S435, R:S434

MW (kDa): 48 kDa

NCBI Gene Symbol: RUNX1

Database Links:
Gene ID: 861
Swiss Prot No.: Q01196
OMIM No.: 151385/601399
Unigene No.: Hs.149261/Hs.612648

Application Dilutions: WB: 1:500~1:1000, ELISA: 1:10000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.