AMV Reverse Transcriptase

AMV Reverse Transcriptase, encoded by Avian Myeloblastosis Virus (AMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template.

AMV Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids. The enzyme possesses an intrinsic RNase H activity. AMV RT possesses multiple enzymatic activities including RNA- and DNA-directed DNA polymerase, DNA-RNA unwinding activity, a sequence- specific Mn2+ -dependent endonuclease and ribonuclease H.

Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of AMLV-RT.

Advantages & Features
- Concentration: 10 U/µL
- Full Solution: includes Reaction Buffer 5x.
- High quality: free of RNase, and DNase (exo- and endonuclease) activities.

Specifications
Unit definition:
One unit is the amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37 °C, using poly(A) oligo dT as a template primer.

Includes
- AMV Reverse Transcriptase (10 U/μL)
- Reaction Buffer 5x

Applications
- RT PCR.
- Synthesis of cDNA.
- RNA Sequencing.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 ºC (NON Frost-Free Freezer).